-media is discarded -sample are unreduced -samples are stored in lysis buffer, Tris-triton ( TC007 ) + protease inhibitor at -20°C -check stock of protease inhibitor !!!! -stored samples are: method trypsin/EDTA & scraping Trypsin/EDTA + scraping EDTA/EGTA & scraping EDTA/EGTA +scraping Scraping in LB Scraping in PBS cell fraction T/S - C T+S - C E/S - C E+S - C CS-LB CS-PBS scraping fraction T/S - S T+S - S E/S - S E+S - S na na sampling seperate together seperate... read more
